نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشکده دندانپزشکی، دانشگاه علوم پزشکی جندی شاپور اهواز، اهواز، ایران
2 مرکز تحقیقات بیمارهای عفونی و گرمسیری خلیج فارس، پژوهشکده سلامت. دانشگاه علوم پزشکی جندی شاپور اهواز، اهواز، ایران.
3 دانشگاه جندی شاپور اهواز
چکیده
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Background: One of the best mouthwashes to reduce dental plaque is chlorhexidine, which is currently the standard anti-plaque treatment. Also, one of the active antimicrobial compounds in the Iranian shallot extract is a flavonoid called Shalomin II, which is known by the formula C14H8O8. The purpose of this study is to compare the antibacterial effect of Shalomine II and Chlorhexidine on Lactobacillus acidophilus and Streptococcus mutans bacteria.
Materials and methods: In this laboratory study, about 300 grams of white shallots were collected from Zagros mountains. Then the aqueous extract was mixed with ethyl acetate (Razi, Iran) at a ratio of 50:50 and mixed for 10 minutes. Microbial resistance and antimicrobial sensitivity of microorganisms were determined using E-test. The bacteria investigated in this study were Lactobacillus acidophilus and Streptococcus mutans. 8 different dilutions were prepared from the combination of half percent Shalomin II, 2 % chlorhexidine and cultured on the bacteria plate. This experiment was repeated three times. Then the plates were incubated at 37°C for 24 hours and examined for growth halo. The location of the closed region was the minimum inhibitory concentration (MIC).
Results: The MIC associated with chlorhexidine against Streptococcus mutans and Lactobacillus acidophilus strains was 0.31 mg/mL and 0.16 mg/mL, respectively, and the MIC associated with Shalomin II against Streptococcus mutans and Lactobacillus acidophilus was 1.95 mg/ml. that was statistically significant (p=0.002). It was also found that the antibacterial effects of chlorhexidine were higher against Lactobacillus acidophilus compared to Streptococcus mutans.
Conclusion: Shalomin II had antimicrobial effects on both bacterial strains, however, had the same inhibitory effect on Lactobacillus acidophilus and Streptococcus mutans. Nevertheless, it has less inhibitory effects than 0.2% chlorhexidine.
کلیدواژه‌ها [English]
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